Identification of Spodoptera Litura Multicapsid Nucleopolyhedrovirus ORF97, A Novel Protein Associated with Envelope of Occlusion-derived Virus
Identifieur interne : 000A03 ( Istex/Checkpoint ); précédent : 000A02; suivant : 000A04Identification of Spodoptera Litura Multicapsid Nucleopolyhedrovirus ORF97, A Novel Protein Associated with Envelope of Occlusion-derived Virus
Auteurs : Weichun Chen [République populaire de Chine] ; Zhaofei Li [République populaire de Chine] ; Sainan Li [République populaire de Chine] ; Lingling Li [République populaire de Chine] ; Kai Yang [République populaire de Chine] ; Yi Pang [République populaire de Chine]Source :
- Virus Genes [ 0920-8569 ] ; 2006-02-01.
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Abstract
Abstract: Open reading frame (ORF) 97 of Spodoptera litura multicapsid nucleopolyhedrovirus (Splt97) is a member of 11k gene family (InterPro database accession number: IPR009313) in insect viruses, potentially encoding 112 amino acids with a predicted molecular weight of 13.2 kDa. Sequence analysis indicated that Splt97 has 8–50% amino acid identity with the homologues of other known baculoviruses including Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) ORF108. Splt97 transcription in SpltMNPV-infected Spli-221 cells was detected from 6 to 96 h postinfection (p.i.) and the mRNA start site was mapped within a consensus baculovirus early promoter motif (ACATT). Time course of Splt97 expression in SpltMNPV-infected cells showed that Splt97 was expressed from 48 through 96 h p.i. as a 13 kDa protein. Western blot analysis revealed that Splt97 was present in occlusion-derived virus (ODV). Furthermore, when ODV was fractionated into nucleocapsid and envelope components, Splt97 was shown to be present only in the envelope. These results suggested that Splt97 was a baculovirus ODV-associated protein.
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DOI: 10.1007/s11262-005-5848-x
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Pang</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:E911D8CD2D34DFF939706E8821FC5805F4998DF4</idno><date when="2006" year="2006">2006</date><idno type="doi">10.1007/s11262-005-5848-x</idno><idno type="url">https://api.istex.fr/ark:/67375/VQC-HLSQF612-3/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000966</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000966</idno><idno type="wicri:Area/Istex/Curation">000966</idno><idno type="wicri:Area/Istex/Checkpoint">000A03</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000A03</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Identification of Spodoptera Litura Multicapsid Nucleopolyhedrovirus ORF97, A Novel Protein Associated with Envelope of Occlusion-derived Virus</title><author><name sortKey="Chen, Weichun" sort="Chen, Weichun" uniqKey="Chen W" first="Weichun" last="Chen">Weichun Chen</name><affiliation wicri:level="3"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Li, Zhaofei" sort="Li, Zhaofei" uniqKey="Li Z" first="Zhaofei" last="Li">Zhaofei Li</name><affiliation wicri:level="3"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Li, Sainan" sort="Li, Sainan" uniqKey="Li S" first="Sainan" last="Li">Sainan Li</name><affiliation wicri:level="3"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Li, Lingling" sort="Li, Lingling" uniqKey="Li L" first="Lingling" last="Li">Lingling Li</name><affiliation wicri:level="3"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Yang, Kai" sort="Yang, Kai" uniqKey="Yang K" first="Kai" last="Yang">Kai Yang</name><affiliation wicri:level="3"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Pang, Yi" sort="Pang, Yi" uniqKey="Pang Y" first="Yi" last="Pang">Yi Pang</name><affiliation wicri:level="3"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">République populaire de Chine</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Virus Genes</title><title level="j" type="abbrev">Virus Genes</title><idno type="ISSN">0920-8569</idno><idno type="eISSN">1572-994X</idno><imprint><publisher>Kluwer Academic Publishers</publisher><pubPlace>Boston</pubPlace><date type="published" when="2006-02-01">2006-02-01</date><biblScope unit="volume">32</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="79">79</biblScope><biblScope unit="page" to="84">84</biblScope></imprint><idno type="ISSN">0920-8569</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0920-8569</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Baculovirus</term><term>ORF97</term><term>Occlusion-derived virus</term><term>Spodoptera litura</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Open reading frame (ORF) 97 of Spodoptera litura multicapsid nucleopolyhedrovirus (Splt97) is a member of 11k gene family (InterPro database accession number: IPR009313) in insect viruses, potentially encoding 112 amino acids with a predicted molecular weight of 13.2 kDa. Sequence analysis indicated that Splt97 has 8–50% amino acid identity with the homologues of other known baculoviruses including Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) ORF108. Splt97 transcription in SpltMNPV-infected Spli-221 cells was detected from 6 to 96 h postinfection (p.i.) and the mRNA start site was mapped within a consensus baculovirus early promoter motif (ACATT). Time course of Splt97 expression in SpltMNPV-infected cells showed that Splt97 was expressed from 48 through 96 h p.i. as a 13 kDa protein. Western blot analysis revealed that Splt97 was present in occlusion-derived virus (ODV). Furthermore, when ODV was fractionated into nucleocapsid and envelope components, Splt97 was shown to be present only in the envelope. These results suggested that Splt97 was a baculovirus ODV-associated protein.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Chen, Weichun" sort="Chen, Weichun" uniqKey="Chen W" first="Weichun" last="Chen">Weichun Chen</name></region><name sortKey="Li, Lingling" sort="Li, Lingling" uniqKey="Li L" first="Lingling" last="Li">Lingling Li</name><name sortKey="Li, Sainan" sort="Li, Sainan" uniqKey="Li S" first="Sainan" last="Li">Sainan Li</name><name sortKey="Li, Zhaofei" sort="Li, Zhaofei" uniqKey="Li Z" first="Zhaofei" last="Li">Zhaofei Li</name><name sortKey="Pang, Yi" sort="Pang, Yi" uniqKey="Pang Y" first="Yi" last="Pang">Yi Pang</name><name sortKey="Pang, Yi" sort="Pang, Yi" uniqKey="Pang Y" first="Yi" last="Pang">Yi Pang</name><name sortKey="Yang, Kai" sort="Yang, Kai" uniqKey="Yang K" first="Kai" last="Yang">Kai Yang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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